Developing mouse VMH Sf1 neurons — female vs male (scRNA-seq)
Mus musculus · Ventromedial hypothalamus · scRNA-seq
Selected data and study scope
This dataset contains scRNA-seq data from Mus musculus, sampled from ventromedial hypothalamus. The default comparison examines gene expression in Male (P10) relative to Female (P10).
The explorer includes 1 track and 1 comparison for this dataset.
The default track, VMH Sf1-lineage neurons (mouse pseudobulk), includes 3 Female (P10) samples and 3 Male (P10) samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The source assay profiles individual cells. The track and sample counts shown here describe the analysis used for the comparison. Cell counts do not replace independent biological replicates.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC passed. Developing mouse VMH Sf1 neurons — female vs male (scRNA-seq); all 1 registered contrasts on default track vmh_sf1
Experimental design passed. Developing mouse VMH Sf1 neurons — female vs male (scRNA-seq); all 1 registered contrasts on default track vmh_sf1
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
FACS-purified Ai14;Sf1-Cre VMH-lineage cells from P10 mice, female n=3 vs male n=3; 467 author-selected VMH cells from 576 deposited wells were aggregated to six mouse pseudobulks. This developmental lineage-enriched contrast is not an adult or whole-hypothalamus estimate (van Veen et al., Nat Metab 2020, PMID 32377634; GSE143818)
Source publication
van Veen JE, Kammel LG, Bunda PC, Shum M, Reid MS, Massa MG, et al.
Nature metabolism · 2020
Repository study title
Estrogen receptor alpha signaling establishes a sexually dimorphic regulatory node of energy expenditure
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Male (P10) vs Female (P10) · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Female (P10) n | Male (P10) n |
|---|---|---|---|
| VMH Sf1-lineage neurons (mouse pseudobulk) | 6,321 | 3 | 3 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.