Mouse arcuate AGRP neurons — fed vs fasting (NuTRAP)
Mus musculus · Arcuate nucleus · TRAP-seq (NuTRAP translatome)
Selected data and study scope
This dataset contains TRAP-seq (NuTRAP translatome) data from Mus musculus, sampled from arcuate nucleus. The default comparison examines gene expression in Fasted relative to Fed.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Arcuate AGRP neurons (NuTRAP), includes 3 Fed samples and 4 Fasted samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
This preparation profiles ribosome-associated RNA from the targeted cells. Treat the result as a translatome measurement. It does not represent total RNA from every cell in the anatomical region.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC passed. Mouse arcuate AGRP neurons — fed vs fasting (NuTRAP); all 1 registered contrasts on default track agrp
Experimental design passed. Mouse arcuate AGRP neurons — fed vs fasting (NuTRAP); all 1 registered contrasts on default track agrp
Author concordance review needed. Matched gene effects; fast_vs_fed. P values and FDR are contextual. Numerical acceptance: strong correlation and matched-gene direction.
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Arcuate AGRP-neuron NuTRAP translatome, ad libitum fed vs overnight fasted; the leptin-injected arm and the POMC-neuron arm are dropped. Note the legacy annotation carries no Igfbp7 or Irs2 feature (Heyward et al., Nat Commun 2024, PMID 38821928; GSE240484)
Source publication
Heyward FD, Liu N, Jacobs C, Machado NLS, Ivison R, Uner A, et al.
Nature communications · 2024
Repository study title
AgRP neuron epigenomes across hunger states reveal that IRF3 mediates leptin's effects
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Fasted vs Fed · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Fed n | Fasted n |
|---|---|---|---|
| Arcuate AGRP neurons (NuTRAP) | 15,242 | 3 | 4 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.