Mouse hypothalamus — 72 h fasting (bulk RNA-seq)
Mus musculus · Whole hypothalamus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Mus musculus, sampled from whole hypothalamus. The default comparison examines gene expression in Fasted 72h relative to Fed.
This comparison examines whole-hypothalamus expression after 72 hours of fasting relative to fed controls. The experiment concerns negative energy balance, rather than diet-induced obesity.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Whole hypothalamus, includes 3 Fed samples and 3 Fasted 72h samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The authors deposited results for fed versus 24-hour fasting and 24-hour versus 72-hour fasting. Their adjacent effect estimates can support a reconstruction of the 72-hour-versus-fed effect. Their P values and FDR cannot be combined into significance values for this comparison.
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC passed. Mouse hypothalamus — 72 h fasting (bulk RNA-seq); all 1 registered contrasts on default track hypothalamus
Experimental design not assessed. Mouse hypothalamus — 72 h fasting (bulk RNA-seq); all 1 registered contrasts on default track hypothalamus
Author concordance not assessed. Reconstructed effects only
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Fed vs 72 h-fasted whole hypothalamus; IGF-transport axis suppressed under fasting — negative energy balance, NOT obesity (GSE294478)
Source publication
Ferris E, Gonzalez Murcia JD, Rodriguez AC, Steinwand S, Stacher Hörndli C, Traenkner D, et al.
Science (New York, N.Y.) · 2025
Repository study title
Genomic Convergence in Hibernating Mammals Elucidates the Genetics of Metabolic Regulation in the Hypothalamus [bulk RNA-seq]
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Fasted 72h vs Fed · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Fed n | Fasted 72h n |
|---|---|---|---|
| Whole hypothalamus | 19,400 | 3 | 3 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.