Human iPSC-derived hypothalamic neurons — inherited obesity
Homo sapiens · Whole hypothalamus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Homo sapiens, sampled from whole hypothalamus. The default comparison examines gene expression in Constitutionally obese donors relative to Healthy control donors.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, iPSC-derived hypothalamic neurons, includes 7 Healthy control donors samples and 5 Constitutionally obese donors samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
These data come from a cell-culture model. Check the cell line or differentiation system and the conditions sampled before interpreting a result.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC passed. Human iPSC-derived hypothalamic neurons — inherited obesity; all 1 registered contrasts on default track hypothalamic_neurons
Experimental design passed. Human iPSC-derived hypothalamic neurons — inherited obesity; all 1 registered contrasts on default track hypothalamic_neurons
Author concordance review needed. Matched gene effects; obesity_vs_control_sex_adjusted. P values and FDR are contextual. No numerical acceptance route has passed for this scope.
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Bulk RNA-seq of iPSC-derived hypothalamic neurons from seven healthy control donors and five constitutionally obese donors, with one independent parent line per library and sex included as an additive covariate. Motor-neuron and postmortem-brain samples are excluded (PMID 29681516; GSE95243)
Source publication
Rajamani U, Gross AR, Hjelm BE, Sequeira A, Vawter MP, Tang J, et al.
Cell stem cell · 2018
Repository study title
Transcriptomes of iPSC-derived and post-mortum Hypothalamus Neurons from obese and control donors
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Constitutionally obese donors vs Healthy control donors · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Healthy control donors n | Constitutionally obese donors n |
|---|---|---|---|
| iPSC-derived hypothalamic neurons | 16,057 | 7 | 5 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.