BL/6 mouse hypothalamic Treg pools — standard diet vs HFD (bulk RNA-seq)
Mus musculus · Whole hypothalamus · bulk RNA-seq (sorted Tregs)
Selected data and study scope
This dataset contains bulk RNA-seq (sorted Tregs) data from Mus musculus, sampled from whole hypothalamus. The default comparison examines gene expression in HFD relative to Std diet.
These data are the deposited BL/6 hypothalamic CD4+CD25+ regulatory T-cell pools under standard diet or high-fat diet. Each library pools cells from five mice.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Hypothalamic Tregs (sorted), includes 4 Std diet samples and 4 HFD samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The displayed sample counts refer to pooled libraries, not individual mice. This cohort differs from the pooled BALB/c Foxp3-GFP cohort displayed in the paper.
The preparation enriches the selected cell population. Interpret expression within that population. Check the source for sorting markers and sample pooling.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC passed. BL/6 mouse hypothalamic Treg pools — standard diet vs HFD (bulk RNA-seq); all 1 registered contrasts on default track treg
Experimental design review needed. BL/6 mouse hypothalamic Treg pools — standard diet vs HFD (bulk RNA-seq); all 1 registered contrasts on default track treg
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Deposited BL/6 hypothalamic CD4+CD25+ Treg pools (five mice per library), standard diet vs HFD; distinct from the paper's displayed pooled BALB/c Foxp3-GFP cohort (Nat Commun 2025, PMID 40113758; GSE282067)
Source publication
Becker M, Kälin S, Neubig AH, Lauber M, Opaleva D, Hipp H, et al.
Nature communications · 2025
Repository study title
Regulatory T-cells in the hypothalamus control immune activation and improve metabolic impairments upon high-calorie environments
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
HFD vs Std diet · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Std diet n | HFD n |
|---|---|---|---|
| Hypothalamic Tregs (sorted) | 5,191 | 4 | 4 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.