Human hypothalamic neural stem cells — hPSC differentiation
Homo sapiens · Whole hypothalamus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Homo sapiens, sampled from whole hypothalamus. The default comparison examines gene expression in Hypothalamic neural stem cells relative to Human pluripotent stem cells.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Hypothalamic neural stem-cell model, includes 3 Human pluripotent stem cells samples and 3 Hypothalamic neural stem cells samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
These data come from a cell-culture model. Check the cell line or differentiation system and the conditions sampled before interpreting a result.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC review needed. Human hypothalamic neural stem cells — hPSC differentiation; all 1 registered contrasts on default track hypothalamic_nsc
Experimental design review needed. Human hypothalamic neural stem cells — hPSC differentiation; all 1 registered contrasts on default track hypothalamic_nsc
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Bulk RNA-seq comparing three human pluripotent-stem-cell cultures with three independently prepared hypothalamus-specific neural-stem-cell cultures. The official StringTie gene-count columns are modeled as a focused in-vitro differentiation contrast (GSE214877)
Repository study title
Hypothalamus-specific NSCs derived from human brain organoids ameliorate age-associated dysfunction upon transplantation into aged mouse hypothalamus [human bulk RNAseq]
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Hypothalamic neural stem cells vs Human pluripotent stem cells · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Human pluripotent stem cells n | Hypothalamic neural stem cells n |
|---|---|---|---|
| Hypothalamic neural stem-cell model | 15,491 | 3 | 3 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.