Chicken POAH — embryonic heat conditioning
Gallus gallus · Preoptic area · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Gallus gallus, sampled from preoptic area. The default comparison examines gene expression in Embryonic heat conditioning relative to Conventional incubation.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Pre-optic anterior hypothalamic area (POAH), includes 4 Conventional incubation samples and 4 Embryonic heat conditioning samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC review needed. Chicken POAH — embryonic heat conditioning; all 1 registered contrasts on default track poah
Experimental design review needed. Chicken POAH — embryonic heat conditioning; all 1 registered contrasts on default track poah
Author concordance review needed. Selected author genes only; ehc_vs_control_poah_hour2_sex_adjusted. P values and FDR are contextual. Numerical acceptance: strong correlation.
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Broiler-chick pre-optic anterior hypothalamic area sampled two hours into an acute post-hatch heat challenge, comparing four conventionally incubated with four embryonically heat-conditioned birds. Sex is balanced and included as an additive covariate; other regions and timepoints are excluded (PMID 40600125; GSE298648)
Source publication
Vaughan R, Sulaiman U, Flynn A, Biase F, Meiri N, Liu D, et al.
Frontiers in physiology · 2025
Repository study title
Embryonic thermal conditioning and post-hatch heat challenge in broiler chicks reveal changes in hypothalamic expression of genes related to appetite, thermoregulation, and stress modulation
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Embryonic heat conditioning vs Conventional incubation · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Conventional incubation n | Embryonic heat conditioning n |
|---|---|---|---|
| Pre-optic anterior hypothalamic area (POAH) | 11,807 | 4 | 4 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.