Rat ventromedial hypothalamus — high-fat diet (bulk RNA-seq)
Rattus norvegicus · Ventromedial hypothalamus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Rattus norvegicus, sampled from ventromedial hypothalamus. The default comparison examines gene expression in High-fat diet relative to Low-fat diet.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Ventromedial hypothalamus (VMH), includes 199 Low-fat diet samples and 192 High-fat diet samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values use variance-stabilized expression (VST). Keep this unit when exporting or comparing values. Values on other normalization scales are not directly interchangeable.
Evidence and verification
Pipeline QC passed. Rat ventromedial hypothalamus — high-fat diet (bulk RNA-seq); all 1 registered contrasts on default track vmh
Experimental design review needed. Rat ventromedial hypothalamus — high-fat diet (bulk RNA-seq); all 1 registered contrasts on default track vmh
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Ventromedial-hypothalamus RNA-seq from 391 independent outbred Heterogeneous Stock rats: 199 low-fat and 192 high-fat animals, balanced across sex and modeled with sex as an additive covariate. The official 28,212-gene VST matrix is analyzed unchanged by limma (GSE330152)
Source publication
Le T, McGee T, Seshie O, Bui T, Giorgio G, Beeson A, et al.
Research square · 2026
Repository study title
Identification of novel candidate neural genes for diet-induced obesity in outbred heterogeneous stock rats
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
High-fat diet vs Low-fat diet · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Low-fat diet n | High-fat diet n |
|---|---|---|---|
| Ventromedial hypothalamus (VMH) | 20,211 | 199 | 192 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.