Mouse VLPO galanin neurons — sleep deprivation
Mus musculus · Preoptic area · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Mus musculus, sampled from preoptic area. The default comparison examines gene expression in Sleep deprivation at ZT6 relative to Spontaneous sleep at ZT6.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, VLPO galanin neurons (LCM), includes 6 Spontaneous sleep at ZT6 samples and 6 Sleep deprivation at ZT6 samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The preparation enriches the selected cell population. Interpret expression within that population. Check the source for sorting markers and sample pooling.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC passed. Mouse VLPO galanin neurons — sleep deprivation; all 1 registered contrasts on default track vlpo_galanin
Experimental design passed. Mouse VLPO galanin neurons — sleep deprivation; all 1 registered contrasts on default track vlpo_galanin
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Galaninergic VLPO-neuron RNA-seq from six spontaneously sleeping and six sleep-deprived mice sampled at the same ZT6 timepoint, with one LCM library per independent animal. Other timepoints and baseline animals are excluded from this focused contrast (PMID 32928100; GSE132308 / PRJNA547578)
Source publication
Guo X, Gao X, Keenan BT, Zhu J, Sarantopoulou D, Lian J, et al.
BMC genomics · 2020
Repository study title
RNA-Seq analysis of galaninergic neurons from ventrolateral preoptic nuleus identifies expression changes between sleep and wake
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Sleep deprivation at ZT6 vs Spontaneous sleep at ZT6 · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Spontaneous sleep at ZT6 n | Sleep deprivation at ZT6 n |
|---|---|---|---|
| VLPO galanin neurons (LCM) | 13,281 | 6 | 6 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.