Mouse paraventricular nucleus — chow vs HFD (bulk RNA-seq)
Mus musculus · Paraventricular nucleus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Mus musculus, sampled from paraventricular nucleus. The default comparison examines gene expression in HFD relative to Chow.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Paraventricular nucleus (PVN), includes 3 Chow samples and 3 HFD samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC passed. Mouse paraventricular nucleus — chow vs HFD (bulk RNA-seq); all 1 registered contrasts on default track pvn
Experimental design passed. Mouse paraventricular nucleus — chow vs HFD (bulk RNA-seq); all 1 registered contrasts on default track pvn
Author concordance review needed. Selected author genes only; hfd_vs_chow. P values and FDR are contextual. Numerical acceptance: strong correlation and matched-gene direction.
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
PVN chow vs HFD — HFD-induced hypothalamic microglial activation / obesity (Yi et al., PMID 35784876; GSE198437)
Source publication
Coker CR, White M, Singal A, Bingaman SS, Paul A, Arnold AC, et al.
Frontiers in physiology · 2022
Repository study title
Hypothalamic Microglia Activation is Associated with High Fat Diet-Induced Obesity and Metabolic Dysfunction in Mice
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
HFD vs Chow · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Chow n | HFD n |
|---|---|---|---|
| Paraventricular nucleus (PVN) | 16,612 | 3 | 3 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.