Mouse hypothalamus — THRB K146Q mutation
Mus musculus · Whole hypothalamus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Mus musculus, sampled from whole hypothalamus. The default comparison examines gene expression in THRB K146Q mutant relative to Wild type.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Whole hypothalamus, includes 3 Wild type samples and 3 THRB K146Q mutant samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values use log2(author-normalized expression + 1). Keep this unit when exporting or comparing values. Values on other normalization scales are not directly interchangeable.
Evidence and verification
Pipeline QC passed. Mouse hypothalamus — THRB K146Q mutation; all 1 registered contrasts on default track hypothalamus
Experimental design passed. Mouse hypothalamus — THRB K146Q mutation; all 1 registered contrasts on default track hypothalamus
Author concordance passed. Matched gene effects; thrb_k146q_vs_wild_type. P values and FDR are contextual. Numerical acceptance: matched-gene direction.
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Whole-hypothalamus RNA-seq from three 3-month-old male THRB K146Q-mutant and three wild-type mice. The six author-normalized sample columns are transformed as log2(x+1) and analyzed by limma without reusing the deposited DE summary (PMID 34237030; GSE167105)
Source publication
Ke S, Liu YY, Karthikraj R, Kannan K, Jiang J, Abe K, et al.
JCI insight · 2021
Repository study title
Thyroid hormone receptor beta is required for thyrotrpin regulation and thyroid hormone production.
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
THRB K146Q mutant vs Wild type · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Wild type n | THRB K146Q mutant n |
|---|---|---|---|
| Whole hypothalamus | 19,773 | 3 | 3 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.