Mouse hypothalamus — HFD time-course (bulk RNA-seq)
Mus musculus · Whole hypothalamus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Mus musculus, sampled from whole hypothalamus. The default comparison examines gene expression in HFD 12w relative to HFD 0d.
This comparison uses the day-0 baseline and the 12-week high-fat-diet samples from the deposited time course.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Whole hypothalamus, includes 6 HFD 0d samples and 6 HFD 12w samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
There is no chow-fed comparison arm in this analysis. The time-course difference should not be described as a matched HFD-versus-chow effect.
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC passed. Mouse hypothalamus — HFD time-course (bulk RNA-seq); all 1 registered contrasts on default track hypothalamus
Experimental design review needed. Mouse hypothalamus — HFD time-course (bulk RNA-seq); all 1 registered contrasts on default track hypothalamus
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
HFD day-0 baseline vs 12 weeks, whole hypothalamus (longitudinal trajectory; no chow arm) (GSE305719)
Repository study title
Dynamical network biomarker analysis using multi-organ RNA sequencing in metabolic syndrome model mice
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
HFD 12w vs HFD 0d · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | HFD 0d n | HFD 12w n |
|---|---|---|---|
| Whole hypothalamus | 14,376 | 6 | 6 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.