Mouse hypothalamus — circadian-rhythm disruption
Mus musculus · Whole hypothalamus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Mus musculus, sampled from whole hypothalamus. The default comparison examines gene expression in 8-week circadian-rhythm disruption relative to 8-week control.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Whole hypothalamus, includes 5 8-week control samples and 5 8-week circadian-rhythm disruption samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values use log2(FPKM + 1). Keep this unit when exporting or comparing values. Values on other normalization scales are not directly interchangeable.
Evidence and verification
Pipeline QC passed. Mouse hypothalamus — circadian-rhythm disruption; all 1 registered contrasts on default track hypothalamus
Experimental design review needed. Mouse hypothalamus — circadian-rhythm disruption; all 1 registered contrasts on default track hypothalamus
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Whole-hypothalamus RNA-seq from five 8-week control and five 8-week circadian-rhythm-disrupted mice. Aged animals and non-hypothalamic tissues are excluded; the official FPKM values are analyzed as documented log2(FPKM+1) without rounding (GSE264350)
Repository study title
Disruption of the Circadian Rhythm Is Exacerbated by Aging and Partially Restored by Time-restricted Feeding
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
8-week circadian-rhythm disruption vs 8-week control · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | 8-week control n | 8-week circadian-rhythm disruption n |
|---|---|---|---|
| Whole hypothalamus | 19,186 | 5 | 5 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.