Mouse hypothalamus — Clic1 knockout (bulk RNA-seq)
Mus musculus · Whole hypothalamus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Mus musculus, sampled from whole hypothalamus. The default comparison examines gene expression in Clic1 knockout relative to Wild type.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Whole hypothalamus, includes 4 Wild type samples and 4 Clic1 knockout samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values use log2(RSEM-normalized expression + 1). Keep this unit when exporting or comparing values. Values on other normalization scales are not directly interchangeable.
Evidence and verification
Pipeline QC review needed. Mouse hypothalamus — Clic1 knockout (bulk RNA-seq); all 1 registered contrasts on default track hypothalamus
Experimental design review needed. Mouse hypothalamus — Clic1 knockout (bulk RNA-seq); all 1 registered contrasts on default track hypothalamus
Author concordance review needed. Matched gene effects; clic1_ko_vs_wild_type. P values and FDR are contextual. No numerical acceptance route has passed for this scope.
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Male-mouse hypothalamus, four unambiguous wild-type vs four Clic1-knockout animals. One metadata-conflicted KO-labelled column and one column without a GEO sample block are excluded rather than guessed (PMID 37604246; GSE229799)
Source publication
Zapata RC, Zhang D, Yoon D, Nasamran CA, Chilin-Fuentes DR, Libster A, et al.
Molecular metabolism · 2023
Repository study title
Targeting Clic1 for the Treatment of Obesity: A Novel Therapeutic Strategy to Reduce Food Intake and Body Weight
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Clic1 knockout vs Wild type · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Wild type n | Clic1 knockout n |
|---|---|---|---|
| Whole hypothalamus | 14,287 | 4 | 4 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.