Mouse hypothalamus — celastrol in DIO (bulk RNA-seq)
Mus musculus · Whole hypothalamus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Mus musculus, sampled from whole hypothalamus. The default comparison examines gene expression in Celastrol relative to Vehicle.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Whole hypothalamus, includes 4 Vehicle samples and 4 Celastrol samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
The measurement combines expression from the cells in the tissue sample. A difference in tissue expression alone does not identify which cell type contributes to it.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC passed. Mouse hypothalamus — celastrol in DIO (bulk RNA-seq); all 1 registered contrasts on default track hypothalamus
Experimental design not assessed. Mouse hypothalamus — celastrol in DIO (bulk RNA-seq); all 1 registered contrasts on default track hypothalamus
Author concordance review needed. Matched gene effects; celastrol_vs_vehicle. P values and FDR are contextual. Numerical acceptance: matched-gene direction.
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Whole hypothalamus from diet-induced-obese C57BL/6J mice after four days of vehicle or celastrol, n=4/4. Pair-fed arms are excluded. The authenticated workbook's integer STRA count sheet—not its separate fractional Cuff sheet—is analyzed with edgeR-QL after recovering Excel date-coerced gene symbols (Guan et al., Cell Metab 2024, PMID 38569472; GSE252779)
Source publication
Guan D, Men Y, Bartlett A, Hernández MAS, Xu J, Yi X, et al.
Cell metabolism · 2024
Repository study title
Central Inhibition of HDAC6 Re-Sensitizes Leptin Signaling During Obesity to Induce Profound Weight Loss
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Celastrol vs Vehicle · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Vehicle n | Celastrol n |
|---|---|---|---|
| Whole hypothalamus | 14,533 | 4 | 4 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.