Mouse PVN sleep-rebound ensembles
Mus musculus · Paraventricular nucleus · bulk RNA-seq
Selected data and study scope
This dataset contains bulk RNA-seq data from Mus musculus, sampled from paraventricular nucleus. The default comparison examines gene expression in Rebound sleep relative to Control sleep.
The explorer includes 1 track and 1 comparison for this dataset.
The default track, Paraventricular nucleus (BacTRAP IP), includes 5 Control sleep samples and 4 Rebound sleep samples in this comparison. These are analysis sample counts. Check the source for biological replication and pooling.
Interpretation and limitations
This preparation profiles ribosome-associated RNA from the targeted cells. Treat the result as a translatome measurement. It does not represent total RNA from every cell in the anatomical region.
Expression values are CPM (TMM-normalized): counts per million on library sizes adjusted by the TMM factors of the study’s own edgeR fit. This scale adjusts for library size and composition, not gene length. Use it to inspect a feature across the samples in this analysis. CPM values are not absolute transcript counts.
Evidence and verification
Pipeline QC passed. Mouse PVN sleep-rebound ensembles; all 1 registered tracks and 1 comparisons
Experimental design review needed. Mouse PVN sleep-rebound ensembles; all 1 registered tracks and 1 comparisons
Author concordance not assessed. Author effect concordance has not been assessed
Pipeline QC can pass while experimental-design limitations remain. Read both assessments before interpreting results.
Open the dataset in the Explorer to read each check and its evidence. Author concordance compares effects for matched genes. It does not require identical author P values or FDR.
Read the analysis methods and interpretation guide and how to cite the source study and HypoExplorer. The source publication may include tissues, groups or comparisons beyond the data shown here.
Source study
Five control and four rebound pools, each containing seven mice. Only immunoprecipitated RNA is compared. Input assays are paired to these pools and excluded from this track.
The original study authors produced these data. HypoExplorer displays selected data and analyses that may cover only part of the study.
Comparisons in HypoExplorer
Rebound vs control sleep · default
Positive differential-expression values indicate higher expression in the case group relative to the baseline. Check the comparison direction and study design when interpreting results.
Available tracks
| Track | Features | Control sleep n | Rebound sleep n |
|---|---|---|---|
| Paraventricular nucleus (BacTRAP IP) | 18,314 | 5 | 4 |
Expression and analysis results
Open this dataset to inspect gene expression and differential-expression results across the available tracks. Pathway enrichment is also available. The pathway databases differ by dataset.
HypoExplorer is in beta testing. Features and analyses may change during testing. Review the original source, analysis methods and quality-control checks before using a result in research.